FREE WEBINAR: Automated direct mRNA sequence mapping using online partial RNase T1 digestion and 2D LC-MS

This webinar highlights a fully automated 2D LC-MS workflow for rapid, high-coverage mRNA characterization. By integrating online partial RNase T1 digestion directly into a 2D LC setup, the method enables controlled, reproducible digestion with seamless transfer into ion-pair reversed-phase separation.

The result:

• 93–99% sequence coverage in <60 minutes
• No post-digest sample handling
• Automated multi-attribute monitoring (sequence identity, 5’ cap, poly(A) tail)

The 2D LC configuration is central to achieving speed, resolution, and robustness in mRNA analysis.

Key Learnings:

• How 2D LC integrates online RNase digestion
• Why 2D LC improves separation and sequence coverage
• Benefits of automation for reproducibility and throughput
• Enabling multi-attribute mRNA analysis in a single workflow

Who should attend?

• mRNA analytics & LC–MS scientists (analytical development, characterization): especially anyone doing mRNA sequence mapping, oligoribonucleotide ID, or improving coverage/speed with LC–MS workflows.
• Biopharma QC / QC method owners working on mRNA identity testing and routine attribute measurements where automation, robustness, and reproducibility matter.
• Process development / CMC / MSAT teams supporting mRNA modalities who need faster turnaround data on critical quality attributes (CQAs) like 5’ cap, poly(A) tail length/heterogeneity, and sequence identity.
• Platform/automation & high-throughput labs looking to reduce hands-on time and eliminate post-digest sample handling while increasing throughput.
• Chromatography / LC system specialists interested in 2D LC configuration, online enzymatic digestion integration, and ion-pair reversed-phase separations for challenging RNA samples.
• R&D leaders / assay strategists evaluating next-gen, multi-attribute monitoring approaches that consolidate multiple measurements into one automated 2D LC–MS method.

Cannot join the broadcast on April 22? Please register anyway to automatically receive access to the recording.

Speaker:

Jessica Dale
PhD Student in Oligonucleotide Analysis, University of Sheffield